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Psd1 Effects on Candida albicans Planktonic Cells and Biofilms.

ABSTRACT: Candida albicans is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) Psd1 is a defensin isolated from Pisum sativum seeds. We tested the effects of this AMP on C. albicans biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three C. albicans variants were studied, one of them a mutant deficient in glucosylceramide synthase, conferring resistance to Psd1 antifungal action. Atomic force microscopy (AFM) was used to assess morphological and biomechanical changes on fungal cells. Surface alterations, with membrane disruption and leakage of cellular contents, were observed. Cytometry assays and confocal microscopy imaging showed that Psd1 causes cell death, in a time and concentration-dependent manner. These results demonstrate Psd1 pleiotropic action against a relevant fungal human pathogen, suggesting its use as natural antimycotic agent.

SUBMITTER: Goncalves S 

PROVIDER: S-EPMC5465278 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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<i>Ps</i>d1 Effects on <i>Candida albicans</i> Planktonic Cells and Biofilms.

Gonçalves Sónia S   Silva Patrícia M PM   Felício Mário R MR   de Medeiros Luciano N LN   Kurtenbach Eleonora E   Santos Nuno C NC  

Frontiers in cellular and infection microbiology 20170609

<i>Candida albicans</i> is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) <i>Ps</i>d1 is a defensin isolated from <i>Pisum sativum</i> seeds. We tested the effects of this AMP on <i>C. albicans</i> biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three <i>C. albicans</i> variants were studied, one of them a mutant def  ...[more]

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