Dataset Information

Amplifying the signal of localized surface plasmon resonance sensing for the sensitive detection of Escherichia coli O157:H7.

ABSTRACT: Gold nanorods (Au NRs) based localized surface plasmon resonance (LSPR) sensors have been widely employed in various fields including biology, environment and food safety detection, but their size- and shape-dependent sensitivity limits their practical applications in sensing and biological detection. In our present work, we proposed an approach to maximally amplify the signal of Au NRs based LSPR sensing by coating an optimized thickness of mesoporous silica onto Au NRs. The plasmonic peaks of Au NRs@SiO₂ with different shell thickness showed finely linear response to the change of surrounding refractive index. The optimized thickness of mesoporous silica of Au NRs@SiO₂ not only provided high stability for LSPR sensor,but also displayed much higher sensitivity (390?nm/RIU) than values of Au NRs from previous reports. The obtained Au NRs@SiO₂ based LSPR sensor was further used in practical application for selectively detection of the E. coli O157:H7, and the detection limit achieved 10 CFU, which is much lower than conventional methods such as electrochemical methods and lateral-flow immunochromatography.

SUBMITTER: Song L

PROVIDER: S-EPMC5468277 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Amplifying the signal of localized surface plasmon resonance sensing for the sensitive detection of Escherichia coli O157:H7.

Song Liping L Zhang Lei L Huang Youju Y Chen Liming L Zhang Ganggang G Shen Zheyu Z Zhang Jiawei J Xiao Zhidong Z Chen Tao T

Scientific reports 20170612 1

Gold nanorods (Au NRs) based localized surface plasmon resonance (LSPR) sensors have been widely employed in various fields including biology, environment and food safety detection, but their size- and shape-dependent sensitivity limits their practical applications in sensing and biological detection. In our present work, we proposed an approach to maximally amplify the signal of Au NRs based LSPR sensing by coating an optimized thickness of mesoporous silica onto Au NRs. The plasmonic peaks of ...[more]

PMID: 28607374

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Project description:The large intestinal pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 detects host cues to regulate virulence gene expression during colonization and infection. However, virulence regulatory mechanisms of EHEC O157:H7 in the human large intestine are not fully understood. Herein, we identified a virulence-regulating pathway where the PhoQ/PhoP two-component regulatory system senses low magnesium levels and signals to the O island 119-encoded Z4267 (LmiA; low magnesium-induced regulator A), directly activating loci of enterocyte effacement genes to promote EHEC O157:H7 adherence in the large intestine. Disruption of this pathway significantly decreased EHEC O157:H7 adherence in the mouse intestinal tract. Moreover, feeding mice a magnesium-rich diet significantly reduced EHEC O157:H7 adherence in vivo This LmiA-mediated virulence regulatory pathway is also conserved among several EHEC and enteropathogenic E. coli serotypes; therefore, our findings support the use of magnesium as a dietary supplement and provide greater insights into the dietary cues that can prevent enteric infections.IMPORTANCE Sensing specific gut metabolites is an important strategy for inducing crucial virulence programs by enterohemorrhagic Escherichia coli (EHEC) O157:H7 during colonization and infection. Here, we identified a virulence-regulating pathway wherein the PhoQ/PhoP two-component regulatory system signals to the O island 119-encoded low magnesium-induced regulator A (LmiA), which, in turn, activates locus of enterocyte effacement (LEE) genes to promote EHEC O157:H7 adherence in the low-magnesium conditions of the large intestine. This regulatory pathway is widely present in a range of EHEC and enteropathogenic E. coli (EPEC) serotypes. Disruption of this pathway significantly decreased EHEC O157:H7 adherence in the mouse intestinal tract. Moreover, mice fed a magnesium-rich diet showed significantly reduced EHEC O157:H7 adherence in vivo, indicating that magnesium may help in preventing EHEC and EPEC infection in humans.

Dataset Information

Amplifying the signal of localized surface plasmon resonance sensing for the sensitive detection of Escherichia coli O157:H7.

Publications

Amplifying the signal of localized surface plasmon resonance sensing for the sensitive detection of Escherichia coli O157:H7.

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