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Fluorescent flavonoids for endoplasmic reticulum cell imaging.


ABSTRACT: Visualization of subcellular organelles in vivo is critical for basic biomedical research and clinical applications. Two new flavonoids with an amide substituent were synthesized and characterized. The flavonoids were nearly non-fluorescent in aqueous environment, but exhibited two emission peaks (one ?em at 495-536 nm and the other at 570-587 nm) in organic solvents, which were assigned to the excited normal (N*) and tautomer (T*) emission. When the dyes were examined on oligodendrocyte cells, they were found to selectively accumulate in the endoplasmic reticulum (ER), a eukaryotic organelle involved in lipid and protein synthesis, giving fluorescence turn-on. The ER-selective flavonoids could be a valuable tool due to its low molecular mass (<500), large Stokes' shift, low toxicity, and biocompatibility.

SUBMITTER: McDonald L 

PROVIDER: S-EPMC5472391 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Fluorescent flavonoids for endoplasmic reticulum cell imaging.

McDonald Lucas L   Liu Bin B   Taraboletti Alexandra A   Whiddon Kyle K   Shriver Leah P LP   Konopka Michael M   Liu Qin Q   Pang Yi Y  

Journal of materials chemistry. B 20161123 48


Visualization of subcellular organelles in vivo is critical for basic biomedical research and clinical applications. Two new flavonoids with an amide substituent were synthesized and characterized. The flavonoids were nearly non-fluorescent in aqueous environment, but exhibited two emission peaks (one <i>λ</i><sub>em</sub> at 495-536 nm and the other at 570-587 nm) in organic solvents, which were assigned to the excited normal (N<sup>*</sup>) and tautomer (T<sup>*</sup>) emission. When the dyes  ...[more]

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