Dataset Information

Dynamics of maternally transferred trace elements in oyster larvae and latent growth effects.

ABSTRACT: Understanding the maternal transfer of contaminants and their potential effects has great implications for a valid ecological assessment of environmental pollution. However, relevant studies on marine bivalves are very limited. Here, we examined the maternal transfer of trace metals in populations of oyster Crassostrea hongkongensis with contrasting metal exposure histories. Elevated accumulation of trace metals was observed in eggs and larvae from contaminated sites, suggesting maternal transfer of multi-metals in natural oyster populations. The dynamics of maternally transferred metals was for the first time documented in this study. We demonstrated that excessively transferred metals in contaminated larvae were rapidly eliminated during the early developmental stage, and the efflux rate of metals in larvae was greatly dependent on environmental contamination level. These results provided the first field evidence of modified metal biokinetics in offsprings due to exposure history of adults in marine bivalves. Moreover, egg production was negatively correlated with the contamination level of metals in eggs. There was a further lagged growth in the contaminated larvae, indicating the potential adverse and latent effects of maternally transferred metals on the viability of oyster offspring. Our findings highlighted the importance of transgenerational studies on long-term metal exposure in marine bivalves.

SUBMITTER: Weng N

PROVIDER: S-EPMC5472574 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Dynamics of maternally transferred trace elements in oyster larvae and latent growth effects.

Weng Nanyan N Wang Wen-Xiong WX

Scientific reports 20170615 1

Understanding the maternal transfer of contaminants and their potential effects has great implications for a valid ecological assessment of environmental pollution. However, relevant studies on marine bivalves are very limited. Here, we examined the maternal transfer of trace metals in populations of oyster Crassostrea hongkongensis with contrasting metal exposure histories. Elevated accumulation of trace metals was observed in eggs and larvae from contaminated sites, suggesting maternal transfe ...[more]

PMID: 28620168

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Project description:Blue-green and brown-spotted eggshells in birds have been proposed as sexual signals of female physiological condition and egg quality, reflecting maternal investment in the egg. Testing this hypothesis requires linking eggshell coloration to egg content, which is lacking for brown protoporphyrin-based pigmentation. As protoporphyrins can induce oxidative stress, and a large amount in eggshells should indicate either high female and egg quality if it reflects the female's high oxidative tolerance, or conversely poor quality if it reflects female physiological stress. Different studies supported either predictions but are difficult to compare given the methodological differences in eggshell-spottiness measurements. Using the blue tit Cyanistes caeruleus as a model species, we aimed at disentangling both predictions in testing if brown-spotted eggshell could reflect the quality of maternal investment in antibodies and carotenoids in the egg, and at improving between-study comparisons in correlating several common measurements of eggshell coloration (spectral and digital measures, spotted surface, pigmentation indices). We found that these color variables were weakly correlated highlighting the need for comparable quantitative measurements between studies and for multivariate regressions incorporating several eggshell-color characteristics. When evaluating the potential signaling function of brown-spotted eggshells, we thus searched for the brown eggshell-color variables that best predicted the maternal transfer of antibodies and carotenoids to egg yolks. We also tested the effects of several parental traits and breeding parameters potentially affecting this transfer. While eggshell coloration did not relate to yolk carotenoids, the eggs with larger and less evenly-distributed spots had higher antibody concentrations, suggesting that both the quantity and distribution of brown pigments reflected the transfer of maternal immune compounds in egg yolks. As yolk antibody concentrations were also positively related to key proxies of maternal quality (egg volume, number, yellow feather brightness, tarsus length), eggshells with larger spots concentrated at their broad pole may indicate higher-quality eggs.

Project description:Several Vibrio spp. cause acute and severe mortality events in hatcheries where larvae of bivalve mollusks are reared, potentially leading to subsequent shortage of bivalve seed for the grow-out industry. In particular, strains of Vibrio coralliilyticus have been identified as a major cause of disease in Pacific, Crassostrea gigas, and eastern, C. virginica, oyster hatcheries in the United States of America. Probiotic bacteria are an inexpensive, practical, and natural method of disease control. Previous research shows that pretreatment of larval oysters with probiotic bacteria Bacillus pumilus RI06-95 (RI) and Phaeobacter inhibens S4 (S4) significantly decreases mortality caused by experimental challenge with the bacterial pathogen V. coralliilyticus RE22 (RE22). This study aims to characterize the immune response of 6-10-day-old eastern oyster larvae to experimental challenge with pathogen V. coralliilyticus RE22 and probionts RI and S4. Treatments included (a) pathogen and probiont exposure at a concentration of 5 × 104 CFU per mL (~2500 bacterial cells per larva) for a duration of 6 h, (b) probiont exposure at the same concentration for a duration of 24 h, and (c) probiont RI daily treatment of larvae in the hatchery for 4, 11, and 15 days. Differential gene expression analysis compared pathogen or probiotic-treated transcriptomes to unexposed controls. Probiotic and pathogen treatment led to upregulation of transcripts coding for several immune pattern recognition receptors (PRRs) involved in environmental sensing and detection of microbes in oyster larvae. Larval oyster responses to pathogen RE22 suggested suppression of expression of genes in immune signaling pathways (myd88, tak1, nkap), failure in upregulation of immune effector genes, high metabolic demand, and oxidative stress that potentially contributed to mortality. On the other hand, the transcriptomic response to probiotic bacteria RI and S4 suggested activation of immune signaling pathways and expression of immune effectors (e.g., Cv-spi2, mucins and perforin-2). These key features of the host immune response to probiotic bacteria were shared despite the length of probiotic exposure, probiotic species, and the type of environment in which exposures were conducted. This study suggests that pre-exposure of eastern oyster larvae to probiotics for 6-24 h prior to pathogenic challenge leads to a robust and effective immune response that may contribute to protecting larvae from subsequent challenge with V. coralliilyticus RE22. This research provides new insights into host-microbe interactions in larval oysters that could be applied in the management of vibriosis in bivalve hatcheries.

Dataset Information

Dynamics of maternally transferred trace elements in oyster larvae and latent growth effects.

Publications

Dynamics of maternally transferred trace elements in oyster larvae and latent growth effects.

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