Ontology highlight
ABSTRACT:
SUBMITTER: Molina RS
PROVIDER: S-EPMC5474692 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Molina Rosana S RS Tran Tam M TM Campbell Robert E RE Lambert Gerard G GG Salih Anya A Shaner Nathan C NC Hughes Thomas E TE Hughes Thomas E TE Drobizhev Mikhail M
The journal of physical chemistry letters 20170525 12
Fluorescent proteins (FPs) are indispensable markers for two-photon imaging of live tissue, especially in the brains of small model organisms. The quantity of physiologically relevant data collected, however, is limited by heat-induced damage of the tissue due to the high intensities of the excitation laser. We seek to minimize this damage by developing FPs with improved brightness. Among FPs with the same chromophore structure, the spectral properties can vary widely due to differences in the l ...[more]