Ontology highlight
ABSTRACT:
SUBMITTER: Arroyo JD
PROVIDER: S-EPMC5474757 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Arroyo Jason D JD Jourdain Alexis A AA Calvo Sarah E SE Ballarano Carmine A CA Doench John G JG Root David E DE Mootha Vamsi K VK
Cell metabolism 20160922 6
Oxidative phosphorylation (OXPHOS) is the major pathway for ATP production in humans. Deficiencies in OXPHOS can arise from mutations in either mitochondrial or nuclear genomes and comprise the largest collection of inborn errors of metabolism. At present we lack a complete catalog of human genes and pathways essential for OXPHOS. Here we introduce a genome-wide CRISPR "death screen" that actively selects dying cells to reveal human genes required for OXPHOS, inspired by the classic observation ...[more]