Dataset Information

Spatio-temporal analysis of coding and long noncoding transcripts during maize endosperm development.

ABSTRACT: The maize endosperm consists of three major compartmentalized cell types: the starchy endosperm (SE), the basal endosperm transfer cell layer (BETL), and the aleurone cell layer (AL). Differential genetic programs are activated in each cell type to construct functionally and structurally distinct cells. To compare gene expression patterns involved in maize endosperm cell differentiation, we isolated transcripts from cryo-dissected endosperm specimens enriched with BETL, AL, or SE at 8, 12, and 16 days after pollination (DAP). We performed transcriptome profiling of coding and long noncoding transcripts in the three cell types during differentiation and identified clusters of the transcripts exhibiting spatio-temporal specificities. Our analysis uncovered that the BETL at 12 DAP undergoes the most dynamic transcriptional regulation for both coding and long noncoding transcripts. In addition, our transcriptome analysis revealed spatio-temporal regulatory networks of transcription factors, imprinted genes, and loci marked with histone H3 trimethylated at lysine 27. Our study suggests that various regulatory mechanisms contribute to the genetic networks specific to the functions and structures of the cell types of the endosperm.

SUBMITTER: Kim ED

PROVIDER: S-EPMC5476630 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Spatio-temporal analysis of coding and long noncoding transcripts during maize endosperm development.

Kim Eun-Deok ED Xiong Yuqing Y Pyo Youngjae Y Kim Dong-Hwan DH Kang Byung-Ho BH Sung Sibum S

Scientific reports 20170619 1

The maize endosperm consists of three major compartmentalized cell types: the starchy endosperm (SE), the basal endosperm transfer cell layer (BETL), and the aleurone cell layer (AL). Differential genetic programs are activated in each cell type to construct functionally and structurally distinct cells. To compare gene expression patterns involved in maize endosperm cell differentiation, we isolated transcripts from cryo-dissected endosperm specimens enriched with BETL, AL, or SE at 8, 12, and 1 ...[more]

PMID: 28630499

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Dataset Information

Spatio-temporal analysis of coding and long noncoding transcripts during maize endosperm development.

Publications

Spatio-temporal analysis of coding and long noncoding transcripts during maize endosperm development.

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