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CD25 + B-1a Cells Express Aicda.


ABSTRACT: B-1a cells are innate-like B-lymphocytes producing natural antibodies. Activation-induced cytidine deaminase (AID), a product of the Aicda gene, plays a central role in class-switch recombination and somatic hypermutation in B cells. Although a role for Aicda in B-1a cells has been suggested on the basis of experiments with knock out (KO) mice, whether B-1a cells express Aicda, and if so, which B-1a cell subpopulation expresses Aicda, remains unknown. Here, we demonstrate that B-1 cells express Aicda, but at a level below that expressed by germinal center (GC) B cells. We previously reported that B-1a cells can be subdivided based on CD25 expression. We show here that B-1a cell Aicda expression is concentrated in the CD25+ B-1a cell subpopulation. These results suggest the possibility that previous studies of memory B cells identified on the basis of Aicda expression may have inadvertently included an unknown number of CD25+ B-1a cells. Although B-1a cells develop normally in the absence of Aicda, a competitive reconstitution assay reveals enhanced vigor for AID KO B-1a cell bone marrow (BM) progenitors, as compared with wild-type BM B-1 cell progenitors. These results suggest that AID inhibits the development of B-1a cells from BM B-1 cell progenitors in a competitive environment.

SUBMITTER: Kaku H 

PROVIDER: S-EPMC5477345 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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CD25 <sup><i>+</i></sup> B-1a Cells Express <i>Aicda</i>.

Kaku Hiroaki H   Holodick Nichol E NE   Tumang Joseph R JR   Rothstein Thomas L TL  

Frontiers in immunology 20170620


B-1a cells are innate-like B-lymphocytes producing natural antibodies. Activation-induced cytidine deaminase (AID), a product of the <i>Aicda</i> gene, plays a central role in class-switch recombination and somatic hypermutation in B cells. Although a role for <i>Aicda</i> in B-1a cells has been suggested on the basis of experiments with knock out (KO) mice, whether B-1a cells express <i>Aicda</i>, and if so, which B-1a cell subpopulation expresses <i>Aicda</i>, remains unknown. Here, we demonst  ...[more]

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