Are we ready to detect nematode diversity by next generation sequencing?
Ontology highlight
ABSTRACT:
SUBMITTER: Peham T
PROVIDER: S-EPMC5478071 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Publications
Are we ready to detect nematode diversity by next generation sequencing?
Peham Thomas T Steiner Florian M FM Schlick-Steiner Birgit C BC Arthofer Wolfgang W
Ecology and evolution 20170427 12
In a Technical Advance article, Porazinska et al. (2009, Molecular Ecology Resources, 9, 1439-1450) assessed next generation sequencing (NGS) as a method for metagenomic analysis of nematode diversity. We agree that NGS has great potential here. However, it is not an easy path to the successful implementation of NGS for environmental DNA analysis of nematodes. Here, we describe the method's limitations and discuss prospective research questions. For instance, only a few direct extraction kits ar ...[more]
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