Ontology highlight
ABSTRACT:
SUBMITTER: Venkat S
PROVIDER: S-EPMC5479488 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Venkat Sumana S Gregory Caroline C Sturges Jourdan J Gan Qinglei Q Fan Chenguang C
Journal of molecular biology 20170331 9
Protein acetylation plays important roles in many biological processes. Malate dehydrogenase (MDH), a key enzyme in the tricarboxylic acid cycle, has been identified to be acetylated in bacteria by proteomic studies, but no further characterization has been reported. One challenge for studying protein acetylation is to get purely acetylated proteins at specific positions. Here, we applied the genetic code expansion strategy to site-specifically incorporate N<sup>ε</sup>-acetyllysine into MDH. Th ...[more]