Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Looking inside the heart: a see-through view of the vascular tree.

ABSTRACT: The ability to acquire 3D images of the heart and its vasculature at cellular resolution facilitates a more detailed study of many heart diseases. Here, we describe a novel technique to image in 3D the heart vasculature by combining the CUBIC clearing protocol combined with in vivo administration of fluorescent-labeled lectin. The use of these techniques in combination with Selective Plane Illumination Microscopy (SPIM) made it possible to obtain high resolution 3D images of the cardiac vascular tree. This methodological approach may enhance the visualization of 3D images of the cardiac vasculature remodeling associated with coronary disease.

SUBMITTER: Nehrhoff I

PROVIDER: S-EPMC5480453 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Looking inside the heart: a see-through view of the vascular tree.

Nehrhoff Imke I Ripoll Jorge J Samaniego Rafael R Desco Manuel M Gómez-Gaviro Maria Victoria MV

Biomedical optics express 20170530 6

The ability to acquire 3D images of the heart and its vasculature at cellular resolution facilitates a more detailed study of many heart diseases. Here, we describe a novel technique to image in 3D the heart vasculature by combining the CUBIC clearing protocol combined with in vivo administration of fluorescent-labeled lectin. The use of these techniques in combination with Selective Plane Illumination Microscopy (SPIM) made it possible to obtain high resolutio ...[more]

PMID: 28663930

Similar Datasets

Looking inside ourselves: a culture of kindness.

Project description:Looking inside ourselves-being present and attentive to our own and others' words and feelings-helps us communicate and interact with a mindful, open heart. Mindfulness and patient-centeredness help caregivers provide higher quality care. Historical background on a predecessor of mindfulness-the biopsychosocial model of health and disease, developed at the University of Rochester-provides context for the mindfulness "movement" in health care. A culture of mindfulness, supported by mindfulness and meditation training for physicians and other health care providers, helps practitioners show greater compassion, kindness, and humanity, all qualities that patients need and deserve. In the health care world, many organizations have been created that focus on aspects of mindfulness. Some have a more clinical emphasis and others focus on behavioral or neuroscience research as it relates to meditation, mindfulness, compassion, and kindness. Mindfulness is also being taught in business schools and corporations. Leaders who approach their teams with respect, integrity, honesty, and kindness are more effective leaders. Organizations like Google, Nike, and Aetna, among others, use the concept of mindfulness, as well as emotional and social intelligence, to build interpersonal competencies and create more people-centered workplaces. As medical libraries live in the health care environment and medical library leaders are key to libraries' present and future, there are strong reasons to address the concepts of mindfulness and kindness and put them to work in the medical library workplaces. A mindfulness meditation exercise closes the lecture, sending the attendees out into their day with calm and open minds and hearts.

| S-EPMC6013138 | biostudies-literature

Neuronal failure in Alzheimer's disease: a view through the oxidative stress looking-glass.

Project description:Considerable debate and controversy surround the cause(s) of Alzheimer's disease (AD). To date, several theories have gained notoriety, however none is universally accepted. In this review, we provide evidence for the oxidative stress-induced AD cascade that posits aged mitochondria as the critical origin of neurodegeneration in AD.

| S-EPMC4097013 | biostudies-other

The Volumetric Source Function: Looking Inside van der Waals Interactions.

Project description:The study of van der Waals interactions plays a central role in the understanding of bonding across a range of biological, chemical and physical phenomena. The presence of van der Waals interactions can be identified through analysis of the reduced density gradient, a fundamental parameter at the core of Density Functional Theory. An extension of Bader's Quantum Theory of Atoms in Molecules is developed here through combination with the analysis of the reduced density gradient. Through this development, a new quantum chemical topological tool is presented: the volumetric source function. This technique allows insight into the atomic composition of van der Waals interactions, offering the first route towards applying the highly successful source function to these disperse interactions. A new algorithm has been implemented in the open-source code, CRITIC2, and tested on acetone, adipic and maleic acids molecular crystals, each stabilized by van der Waals interactions. This novel technique for studying van der Waals interactions at an atomic level offers unprecedented opportunities in the fundamental study of intermolecular interactions and molecular design for crystal engineering, drug design and bio-macromolecular processes.

| S-EPMC7210285 | biostudies-literature

Looking Back and Looking Around: How Athletes, Parents and Coaches See Psychosocial Development in Adolescent Performance Sport.

Project description:Sport has the potential to support psychosocial development in young people. However, extant studies have tended to evaluate purpose-built interventions, leaving regular organised sport relatively overlooked. Moreover, previous work has tended to concentrated on a narrow range of outcomes. To address these gaps, we conducted a season-long ethnography of a youth performance sport club based on a novel Realist Evaluation approach. We construed the club as a social intervention within a complex system of agents and structures. The results are published in this special issue as a two-part series. In this first paper, we detail the perceptions of former and current club parents, players and coaches, using them to build a set of programme theories. The resulting network of outcomes (i.e., self, emotional, social, moral and cognitive) and generative mechanisms (i.e., the attention factory, the greenhouse for growth, the personal boost and the real-life simulator), spanning across multiple contextual layers, provides a nuanced understanding of stakeholders' views and experiences. This textured perspective of the multi-faceted process of development provides new insights for administrators, coaches and parents to maximise the developmental properties of youth sport, and signposts new avenues for research in this area.

| S-EPMC9030748 | biostudies-literature

An inside view: VEGF receptor trafficking and signaling.

Project description:Vascular endothelial growth factors (VEGF) and their receptors play a central role in the development of cardiovascular system and in vasculature-related processes in the adult organism. Given the critical role of this signaling cascade, intricate control systems have evolved to regulate its function. A new layer of added complexity has been the demonstration of the importance of endocytosis and intracellular trafficking of VEGF receptors in the regulation of VEGF signaling. In this review, we consider an evolving link between VEGF receptor endocytosis, trafficking, and signaling and their biological function.

| S-EPMC4037811 | biostudies-literature

Waves of calcium depletion in the sarcoplasmic reticulum of vascular smooth muscle cells: an inside view of spatiotemporal Ca2+ regulation.

Project description:Agonist-stimulated smooth muscle Ca2+ waves regulate blood vessel tone and vasomotion. Previous studies employing cytoplasmic Ca2+ indicators revealed that these Ca2+ waves were stimulated by a combination of inositol 1,4,5-trisphosphate- and Ca2+ -induced Ca2+ release from the endo/sarcoplasmic reticulum. Herein, we present the first report of endothelin-1 stimulated waves of Ca2+ depletion from the sarcoplasmic reticulum of vascular smooth muscle cells using a calsequestrin-targeted Ca2+ indicator. Our findings confirm that these waves are due to regenerative Ca2+ -induced Ca2+ release by the receptors for inositol 1,4,5-trisphosphate. Our main new finding is a transient elevation in SR luminal Ca2+ concentration ([Ca2+](SR)) both at the site of wave initiation, just before regenerative Ca2+ release commences, and at the advancing wave front, during propagation. This strongly suggests a role for [Ca2+](SR) in the activation of inositol 1,4,5-trisphosphate receptors during agonist-induced calcium waves. In addition, quantitative analysis of the gradual decrease in the velocity of the depletion wave, observed in the absence of external Ca2+, indicates continuity of the lumen of the sarcoplasmic reticulum network. Finally, our observation that the depletion wave was arrested by the nuclear envelope may have implications for selective Ca2+ signalling.

| S-EPMC3567057 | biostudies-literature

Looking Inside the World of Granulosa Cells: The Noxious Effects of Cigarette Smoke.

Project description:The detrimental implications of tobacco smoke on systemic health have been widely established during the past few decades. Nonetheless, increasing evidence has begun to shed more light on the serious impact that smoke exposure could also have on mammal reproductive health in terms of overall ovarian dysfunction and gestation. A variety of these complications seem to be causally related to specific chemical substances contained in cigarette smoke and their possible effects on ovarian tissues and cells, such as granulosa cells. Granulosa cells represent the functional unit of the ovary and are able to establish a bidirectional cross-talk relationship with the oocyte during folliculogenesis, which makes them vital for its correct growth and development. Based on these premises, the current review focuses on the presence of related smoke-induced damages in granulosa cells. Data have been grouped according to the studied tobacco constituents and the molecular pathways involved, in order to synthesize their impact on granulosa cells and fertility. Attention is further brought to the correlation between electronic cigarettes and female reproduction, although there have been no investigations so far regarding e-cigarette-related granulosa cell exposure. We summarize how tobacco constituents are able to cause alterations in the "life" of granulosa cells, ranging from luteal steroidogenesis and follicular loss to granulosa cell apoptosis and activation of the autophagic machinery. Further studies have been conducted to elucidate the relationship between lifestyle and fertility as to reduce the morbidity connected with infertility.

| S-EPMC7555008 | biostudies-literature

Looking inside the Ocean: Toward an Autonomous Imaging System for Monitoring Gelatinous Zooplankton.

Project description:Marine plankton abundance and dynamics in the open and interior ocean is still an unknown field. The knowledge of gelatinous zooplankton distribution is especially challenging, because this type of plankton has a very fragile structure and cannot be directly sampled using traditional net based techniques. To overcome this shortcoming, Computer Vision techniques can be successfully used for the automatic monitoring of this group.This paper presents the GUARD1 imaging system, a low-cost stand-alone instrument for underwater image acquisition and recognition of gelatinous zooplankton, and discusses the performance of three different methodologies, Tikhonov Regularization, Support Vector Machines and Genetic Programming, that have been compared in order to select the one to be run onboard the system for the automatic recognition of gelatinous zooplankton. The performance comparison results highlight the high accuracy of the three methods in gelatinous zooplankton identification, showing their good capability in robustly selecting relevant features. In particular, Genetic Programming technique achieves the same performances of the other two methods by using a smaller set of features, thus being the most efficient in avoiding computationally consuming preprocessing stages, that is a crucial requirement for running on an autonomous imaging system designed for long lasting deployments, like the GUARD1. The Genetic Programming algorithm has been installed onboard the system, that has been operationally tested in a two-months survey in the Ligurian Sea, providing satisfactory results in terms of monitoring and recognition performances.

| S-EPMC5191104 | biostudies-literature

Looking for trees in the forest: summary tree from posterior samples.

Project description:BackgroundBayesian phylogenetic analysis generates a set of trees which are often condensed into a single tree representing the whole set. Many methods exist for selecting a representative topology for a set of unrooted trees, few exist for assigning branch lengths to a fixed topology, and even fewer for simultaneously setting the topology and branch lengths. However, there is very little research into locating a good representative for a set of rooted time trees like the ones obtained from a BEAST analysis.ResultsWe empirically compare new and known methods for generating a summary tree. Some new methods are motivated by mathematical constructions such as tree metrics, while the rest employ tree concepts which work well in practice. These use more of the posterior than existing methods, which discard information not directly mapped to the chosen topology. Using results from a large number of simulations we assess the quality of a summary tree, measuring (a) how well it explains the sequence data under the model and (b) how close it is to the "truth", i.e to the tree used to generate the sequences.ConclusionsOur simulations indicate that no single method is "best". Methods producing good divergence time estimates have poor branch lengths and lower model fit, and vice versa. Using the results presented here, a user can choose the appropriate method based on the purpose of the summary tree.

| S-EPMC3853548 | biostudies-literature

Development of radioimmunotherapeutic and diagnostic antibodies: an inside-out view.

Project description:Only a handful of radiolabeled antibodies (Abs) have gained US Food and Drug Administration (FDA) approval for use in clinical oncology, including four immunodiagnostic agents and two targeted radioimmunotherapeutic agents. Despite the advent of nonimmunogenic Abs and the availability of a diverse library of radionuclides, progress beyond early Phase II radioimmunotherapy (RIT) studies in solid tumors has been marginal. Furthermore, [18F]fluorodeoxyglucose continues to dominate the molecular imaging domain, underscored by a decade-long absence of any newly approved Ab-based imaging agent (none since 1996). Why has the development of clinically successful Abs for RIT been limited to lymphoma? What obstacles must be overcome to allow the FDA approval of immuno-positron emission tomography (immuno-PET) imaging agents? How can we address the unique challenges that have thus far prevented the introduction of Ab-based imaging agents and therapeutics for solid tumors? Many poor decisions have been made regarding radiolabeled Abs, but useful insight can be gained from these mistakes. The following review addresses the physical, chemical, biological, clinical, regulatory and financial limitations that impede the progress of this increasingly important class of drugs.

| S-EPMC2212602 | biostudies-other

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data