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Biomimetic Molecular Signaling using DNA Walkers on Microparticles.

ABSTRACT: We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or "track") for the molecular walker. We distinguish these particle types using fluorescence barcoding: we synthesized and distinguished multiple particle types with multicolor fluorescence microscopy and automated image analysis software. This represents a step toward amplified, multiplex, and microscopically localized detection based on DNA nanotechnology.

SUBMITTER: Damase TR

PROVIDER: S-EPMC5481426 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Biomimetic Molecular Signaling using DNA Walkers on Microparticles.

Damase Tulsi Ram TR Spencer Adam A Samuel Bamidele B Allen Peter B PB

Scientific reports 20170622 1

We report the release of catalytic DNA walkers from hydrogel microparticles and the detection of those walkers by substrate-coated microparticles. This might be considered a synthetic biology analog of molecular signal release and reception. One type of particles was coated with components of a DNA one-step strand displacement (OSD) reaction to release the walker. A second type of particle was coated with substrate (or "track") for the molecular walker. We distinguish these particle types using ...[more]

PMID: 28642488

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