Ontology highlight
ABSTRACT:
SUBMITTER: He E
PROVIDER: S-EPMC5482055 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
He Enqi E Wierda Keimpe K van Westen Rhode R Broeke Jurjen H JH Toonen Ruud F RF Cornelisse L Niels LN Verhage Matthijs M
Nature communications 20170621
Synaptic transmission requires a stable pool of release-ready (primed) vesicles. Here we show that two molecules involved in SNARE-complex assembly, Munc13-1 and Munc18-1, together stabilize release-ready vesicles by preventing de-priming. Replacing neuronal Munc18-1 by a non-neuronal isoform Munc18-2 (Munc18-1/2SWAP) supports activity-dependent priming, but primed vesicles fall back into a non-releasable state (de-prime) within seconds. Munc13-1 deficiency produces a similar defect. Inhibitors ...[more]