Ontology highlight
ABSTRACT:
SUBMITTER: Horejsh D
PROVIDER: S-EPMC548373 | biostudies-literature | 2005
REPOSITORIES: biostudies-literature
Horejsh Douglas D Martini Federico F Poccia Fabrizio F Ippolito Giuseppe G Di Caro Antonino A Capobianchi Maria R MR
Nucleic acids research 20050119 2
Molecular beacons are dual-labelled probes that are typically used in real-time PCR assays, but have also been conjugated with solid matrices for use in microarrays or biosensors. We have developed a fluid array system using microsphere-conjugated molecular beacons and the flow cytometer for the specific, multiplexed detection of unlabelled nucleic acids in solution. For this array system, molecular beacons were conjugated with microspheres using a biotin-streptavidin linkage. A bridged conjugat ...[more]