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In vivo IL-4 prevents allo-antigen driven CD8+ CTL development.

ABSTRACT: IL-4 has been shown to suppress acute graft vs. host disease (GVHD) in irradiated hosts. Here we evaluated whether IL-4 suppresses acute GVHD in the un-irradiated parent-into-F1 GVHD model with relevance to renal allograft rejection. IL-4 completely suppressed CD8 CTL when administered with donor cells however this effect was lost if its administration was delayed 3days. IL-4 did not inhibit donor CD8+ T cell homing to the host spleen but rather prevented donor CD8+ T cell differentiation into CTLs. Studies with IL-4R?-deficient donor cells or recipient mice demonstrated that IL-4 effects on the host, rather than, or in addition to IL-4 effects on donor cells, were critical for suppression of CTL. Because IL-4 decreased all splenic dendritic cell populations and increased neutrophil and CD8+ T cells, IL-4 may suppress donor CD8+ CTL by decreasing Ag presentation and/or increasing host myeloid and CD8+ T cell suppression of donor T cells.

SUBMITTER: Via CS

PROVIDER: S-EPMC5484589 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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In vivo IL-4 prevents allo-antigen driven CD8+ CTL development.

Via Charles S CS Soloviova Kateryna K Puliaiev Maksym M Puliav Roman R Puliaeva Irina I Morris Suzanne C SC Finkelman Fred D FD

Clinical immunology (Orlando, Fla.) 20170327

IL-4 has been shown to suppress acute graft vs. host disease (GVHD) in irradiated hosts. Here we evaluated whether IL-4 suppresses acute GVHD in the un-irradiated parent-into-F1 GVHD model with relevance to renal allograft rejection. IL-4 completely suppressed CD8 CTL when administered with donor cells however this effect was lost if its administration was delayed 3days. IL-4 did not inhibit donor CD8+ T cell homing to the host spleen but rather prevented donor CD8+ T cell ...[more]

PMID: 28359782

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