C-Myc is Required for BRAF^V600E-Induced Epigenetic Silencing by H3K27me3 in Tumorigenesis.

ABSTRACT: BRAF^V600E mutation is frequently found in human cancers particularly thyroid cancer and melanoma, and is involved in the regulation of gene expression through activating MAPK/Erk signaling. Trimethylation of histone 3 lysine 27 (H3K27me3) is a critical epigenetic mark for the maintenance of gene silencing in tumorigenesis. However, molecular mechanism underlying the complex interplay between these two molecular events remains to be explored. In the present study, we conducted chromatin immunoprecipitation combined with next-generation sequencing (ChIP-Seq) and expression microarray analysis in NIH3T3 cells to explore the relationship between H3K27me3 and transcriptional regulation by BRAF^V600E mutation. Our results showed that activated MAPK/Erk signalin

SUBMITTER: Qu Y 

PROVIDER: S-EPMC5485424 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature