One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes.

ABSTRACT: Neural cell fate is determined by a tightly controlled transcription regulatory network during development. The ability to manipulate the expression of multiple transcription factors simultaneously is required to delineate the complex picture of neural cell development. Because of the limited carrying capacity of the commonly used viral vectors, such as lentiviral or retroviral vectors, it is often challenging to perform perturbation experiments on multiple transcription factors. Here we have developed a piggyBac (PB) transposon-based CRISPR activation (CRISPRa) all-in-one system, which allows for simultaneous and stable endogenous transactivation of multiple transcription factors and long non-coding RNAs. As a proof of principle, we showed that the PB-CRISPRa system could accelerate the d

SUBMITTER: Li S 

PROVIDER: S-EPMC5485764 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature