Ontology highlight
ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC3969854 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Cell 20130502 4
Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been adapted as an efficient gene-targeting technology with the potential for multiplexed genome editing. We demonstrate that CRISPR/Cas-mediated gene editing allows the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty--8 alleles) in mouse embryonic stem (ES) cells with hi ...[more]