Ontology highlight
ABSTRACT:
SUBMITTER: Hedges J
PROVIDER: S-EPMC548654 | biostudies-literature | 2005 Feb
REPOSITORIES: biostudies-literature
Hedges John J West Matthew M Johnson Arlen W AW
The EMBO journal 20050120 3
In eukaryotes, nuclear export of the large (60S) ribosomal subunit requires the adapter protein Nmd3p to provide the nuclear export signal. Here, we show that in yeast release of Nmd3p from 60S subunits in the cytoplasm requires the ribosomal protein Rpl10p and the G-protein, Lsg1p. Mutations in LSG1 or RPL10 blocked Nmd3-GFP shuttling into the nucleus and export of pre-60S subunits from the nucleus. Overexpression of NMD3 alleviated the export defect, indicating that the block in 60S export in ...[more]