Ontology highlight
ABSTRACT:
SUBMITTER: Bekker-Jensen DB
PROVIDER: S-EPMC5493283 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Bekker-Jensen Dorte B DB Kelstrup Christian D CD Batth Tanveer S TS Larsen Sara C SC Haldrup Christa C Bramsen Jesper B JB Sørensen Karina D KD Høyer Søren S Ørntoft Torben F TF Andersen Claus L CL Nielsen Michael L ML Olsen Jesper V JV
Cell systems 20170607 6
This study investigates the challenge of comprehensively cataloging the complete human proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics. We modify a classical two-dimensional high-resolution reversed-phase peptide fractionation scheme and optimize a protocol that provides sufficient peak capacity to saturate the sequencing speed of modern MS instruments. This strategy enables the deepest proteome of a human single-cell type to date, with the HeLa proteome se ...[more]