Ontology highlight
ABSTRACT:
SUBMITTER: Ohta H
PROVIDER: S-EPMC5494472 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Ohta Hiroshi H Kurimoto Kazuki K Okamoto Ikuhiro I Nakamura Tomonori T Yabuta Yukihiro Y Miyauchi Hidetaka H Yamamoto Takuya T Okuno Yukiko Y Hagiwara Masatoshi M Shirane Kenjiro K Sasaki Hiroyuki H Saitou Mitinori M
The EMBO journal 20170530 13
The expansion of primordial germ cells (PGCs), the precursors for the oocytes and spermatozoa, is a key challenge in reproductive biology/medicine. Using a chemical screening exploiting PGC-like cells (PGCLCs) induced from mouse embryonic stem cells (ESCs), we here identify key signaling pathways critical for PGCLC proliferation. We show that the combinatorial application of Forskolin and Rolipram, which stimulate cAMP signaling via different mechanisms, expands PGCLCs up to ~50-fold in culture. ...[more]