Unknown

Dataset Information

0

Protein-bound polyphenols create "ghost" band artifacts during chemiluminescence-based antigen detection.


ABSTRACT: Antigen detection during Western blotting commonly utilizes a horseradish peroxidase-coupled secondary antibody and enhanced chemiluminescent substrate. We utilized this technique to examine the impact of green tea-derived polyphenols on the binding of egg white protein-specific IgE antibodies from allergic human plasma to their cognate antigens. Our experiments unexpectedly showed that green tea-derived polyphenols, when stably complexed with egg white proteins, caused "ghost" band formation in the presence of horseradish peroxide. This study suggests that caution should be taken when evaluating polyphenol-bound proteins by enhanced chemiluminescence Western blotting using horseradish peroxidase and demonstrates that protein-bound polyphenols can be a source of "ghost" band artifacts on Western blots.

SUBMITTER: Plundrich N 

PROVIDER: S-EPMC5497812 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3973438 | biostudies-literature
| S-EPMC7661926 | biostudies-literature
| S-EPMC6651840 | biostudies-literature
| S-EPMC8038133 | biostudies-literature
| S-EPMC3697675 | biostudies-literature
| S-EPMC6277231 | biostudies-literature
| S-EPMC8557186 | biostudies-literature
| S-EPMC5656605 | biostudies-literature
| S-EPMC6820691 | biostudies-literature
| S-EPMC4574305 | biostudies-literature