Ontology highlight
ABSTRACT:
SUBMITTER: Adikusuma F
PROVIDER: S-EPMC5500146 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Adikusuma Fatwa F Pederick Daniel D McAninch Dale D Hughes James J Thomas Paul P
Genetics 20170517 3
Gene duplication provides spare genetic material that evolution can craft into new functions. <i>Sox2</i> and <i>Sox3</i> are evolutionarily related genes with overlapping and unique sites of expression during embryogenesis. It is currently unclear whether SOX2 and SOX3 have identical or different functions. Here, we use CRISPR/Cas9-assisted mutagenesis to perform a gene-swap, replacing the <i>Sox3</i> ORF with the <i>Sox2</i> ORF to investigate their functional equivalence in the brain and test ...[more]