Ontology highlight
ABSTRACT:
SUBMITTER: Takeuchi H
PROVIDER: S-EPMC5500366 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Takeuchi Hiroaki H Saito Hideki H Noda Takeshi T Miyamoto Tadashi T Yoshinaga Tomokazu T Terahara Kazutaka K Ishii Hiroshi H Tsunetsugu-Yokota Yasuko Y Yamaoka Shoji S
PLoS pathogens 20170706 7
Regulation of capsid disassembly is crucial for efficient HIV-1 cDNA synthesis after entry, yet host factors involved in this process remain largely unknown. Here, we employ genetic screening of human T-cells to identify maternal embryonic leucine zipper kinase (MELK) as a host factor required for optimal uncoating of the HIV-1 core to promote viral cDNA synthesis. Depletion of MELK inhibited HIV-1 cDNA synthesis with a concomitant delay of capsid disassembly. MELK phosphorylated Ser-149 of the ...[more]