Ontology highlight
ABSTRACT:
SUBMITTER: Marquez CL
PROVIDER: S-EPMC6039174 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Márquez Chantal L CL Lau Derrick D Walsh James J Shah Vaibhav V McGuinness Conall C Wong Andrew A Aggarwal Anupriya A Parker Michael W MW Jacques David A DA Turville Stuart S Böcking Till T
eLife 20180607
Uncoating of the metastable HIV-1 capsid is a tightly regulated disassembly process required for release of the viral cDNA prior to nuclear import. To understand the intrinsic capsid disassembly pathway and how it can be modulated, we have developed a single-particle fluorescence microscopy method to follow the real-time uncoating kinetics of authentic HIV capsids in vitro immediately after permeabilizing the viral membrane. Opening of the first defect in the lattice is the rate-limiting step of ...[more]