Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS.

ABSTRACT: The observation of molecular diffusion at different spatial scales, and in particular below the optical diffraction limit (<200 nm), can reveal details of the subcellular topology and its functional organization. Stimulated-emission depletion microscopy (STED) has been previously combined with fluorescence correlation spectroscopy (FCS) to investigate nanoscale diffusion (STED-FCS). However, stimulated-emission depletion fluorescence correlation spectroscopy has only been used successfully to reveal functional organization in two-dimensional space, such as the plasma membrane, while, an efficient implementation for measurements in three-dimensional space, such as the cellular interior, is still lacking. Here we integrate the STED-FCS method with two analytical approaches, the recent separa

SUBMITTER: Lanzano L 

PROVIDER: S-EPMC5500520 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature