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HiChIP: efficient and sensitive analysis of protein-directed genome architecture.

ABSTRACT: Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

SUBMITTER: Mumbach MR

PROVIDER: S-EPMC5501173 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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HiChIP: efficient and sensitive analysis of protein-directed genome architecture.

Mumbach Maxwell R MR Rubin Adam J AJ Flynn Ryan A RA Dai Chao C Khavari Paul A PA Greenleaf William J WJ Chang Howard Y HY

Nature methods 20160919 11

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C. ...[more]

PMID: 27643841

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HiChIP: Efficient and sensitive analysis of protein-directed genome architecture

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