Ontology highlight
ABSTRACT:
SUBMITTER: Mumbach MR
PROVIDER: S-EPMC5501173 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Mumbach Maxwell R MR Rubin Adam J AJ Flynn Ryan A RA Dai Chao C Khavari Paul A PA Greenleaf William J WJ Chang Howard Y HY
Nature methods 20160919 11
Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C. ...[more]