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HiChIP: Efficient and sensitive analysis of protein-directed genome architecture

ABSTRACT: Three-dimensional genome structure is central to gene control, but current technologies are often impractical for many biological questions due to cell and read number requirements. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the fraction of conformation-informative reads by over 10-fold and lowers input requirement greater than 100-fold to 1 million cells. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to noise than in situ Hi-C. Thus, HiChIP will enable insight into the genome’s three-dimensional structure and regulation in once-inaccessible biological systems.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE80820 | GEO | 2016/08/31

SECONDARY ACCESSION(S): PRJNA320090

REPOSITORIES: GEO

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