Ontology highlight
ABSTRACT:
SUBMITTER: Chendeb M
PROVIDER: S-EPMC5503529 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Chendeb Mariam M Schneider Robert R Davidson Irwin I Fadloun Anas A
Oncotarget 20170601 24
In gene therapy, effective and selective suicide gene expression is crucial. We exploited the endogenous Long INterspersed Element-1 (L1) machinery often reactivated in human cancers to integrate the Herpes Simplex Virus Thymidine Kinase (HSV-TK) suicide gene selectively into the genome of cancer cells. We developed a plasmid-based system directing HSV-TK expression only when reverse transcribed and integrated in the host genome via the endogenous L1 ORF1/2 proteins and an Alu element. Delivery ...[more]