Potentiation of receptor responses induced by prolonged binding of Gα₁₃ and leukemia-associated RhoGEF.

ABSTRACT: Diverse cellular functions are controlled by RhoA-GTPases, which are activated by trimeric G proteins via RhoGEFs, among others. In this study, we focused on the signaling from GPCRs to RhoA via Gα₁₃ and leukemia-associated RhoGEF (LARG). The activation of Gα₁₃ was elucidated in living cells with high temporal and spatial resolution by means of FRET. The inactivation after agonist withdrawal occurred in the same range (t_1/2 = 25.3 ± 2.2 s; mean ± sem; n = 22) as described for other Gα proteins. The interaction of Gα₁₃ and LARG and the thereby-induced LARG translocation to the plasma membrane were at least 1 order of magnitude more stable after agonist withdrawal, exceeding Gα₁₃ deactivation in the absence of

SUBMITTER: Bodmann EL 

PROVIDER: S-EPMC5503709 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature