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2'-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA.

ABSTRACT: 5-Hydroxymethylcytidine (hm⁵C) was recently identified as a direct metabolite of m⁵C in RNA. We investigated the stability of hm⁵C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m⁵C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm⁵Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.

SUBMITTER: Huber SM

PROVIDER: S-EPMC5504488 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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2'-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA.

Huber Sabrina M SM van Delft Pieter P Tanpure Arun A Miska Eric A EA Balasubramanian Shankar S

Journal of the American Chemical Society 20170125 5

5-Hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this ...[more]

PMID: 28107630

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