Synthesis and Multiple Incorporations of 2'-O-Methyl-5-hydroxymethylcytidine, 5-Hydroxymethylcytidine and 5-Formylcytidine Monomers into RNA Oligonucleotides.

ABSTRACT: The synthesis of 2'-O-methyl-5-hydroxymethylcytidine (hm⁵ Cm), 5-hydroxymethylcytidine (hm⁵ C) and 5-formylcytidine (f⁵ C) phosphoramidite monomers has been developed. Optimisation of mild post-synthetic deprotection conditions enabled the synthesis of RNA containing all four naturally occurring cytosine modifications (hm⁵ Cm, hm⁵ C, f⁵ C plus 5-methylcytosine). Given the considerable interest in RNA modifications and epitranscriptomics, the availability of synthetic monomers and RNAs containing these modifications will be valuable for elucidating their biological function(s).

SUBMITTER: Tanpure AA 

PROVIDER: S-EPMC5779611 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature