Ontology highlight
ABSTRACT:
SUBMITTER: Bijnsdorp IV
PROVIDER: S-EPMC5505003 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Bijnsdorp Irene V IV Maxouri Olga O Kardar Aarzo A Schelfhorst Tim T Piersma Sander R SR Pham Thang V TV Vis Andre A van Moorselaar R Jeroen RJ Jimenez Connie R CR
Journal of extracellular vesicles 20170428 1
Extracellular vesicles (EVs) secreted by prostate cancer (PCa) cells contain specific biomarkers and can be isolated from urine. Collection of urine is not invasive, and therefore urinary EVs represent a liquid biopsy for diagnostic and prognostic testing for PCa. In this study, we optimised urinary EV isolation using a method based on heat shock proteins and compared it to gold-standard ultracentrifugation. The urinary EV isolation protocol using the Vn96-peptide is easier, time convenient (≈1. ...[more]