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A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom.

ABSTRACT: Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10?kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4?h), trypsin (18?h), pepsin (18?h), and chymotrypsin (18?h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523.

SUBMITTER: Trevisan-Silva D 

PROVIDER: S-EPMC5505115 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom.

Trevisan-Silva Dilza D   Bednaski Aline V AV   Fischer Juliana S G JSG   Veiga Silvio S SS   Bandeira Nuno N   Guthals Adrian A   Marchini Fabricio K FK   Leprevost Felipe V FV   Barbosa Valmir C VC   Senff-Ribeiro Andrea A   Carvalho Paulo C PC  

Scientific data 20170711

Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotr  ...[more]

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