Dataset Information

A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

ABSTRACT: Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom of 200 spiders was extracted and fractioned into aliquots greater than or less than 10kDa. Then, each aliquot was further aliquoted and digested with trypsin (4h), trypsin (18h), Pepsin (18h), Chymotrypsin (18h), and analyzed by MudPIT on an Orbitrap XL mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain de meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of this venom.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Loxosceles Intermedia

SUBMITTER: Paulo Carvalho

LAB HEAD: Paulo Costa Carvalho

PROVIDER: PXD005523 | Pride | 2017-05-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20130109_PC_VN_SCX100_maior10_P_1.RAW	Raw
	20130109_PC_VN_SCX100_maior10_P_2.RAW	Raw
	20130109_PC_VN_SCX100_maior10_Q_1.RAW	Raw
	20130109_PC_VN_SCX100_maior10_Q_2.RAW	Raw
	20130109_PC_VN_SCX100_maior10_T18_1.RAW	Raw

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A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom.

Trevisan-Silva Dilza D Bednaski Aline V AV Fischer Juliana S G JSG Veiga Silvio S SS Bandeira Nuno N Guthals Adrian A Marchini Fabricio K FK Leprevost Felipe V FV Barbosa Valmir C VC Senff-Ribeiro Andrea A Carvalho Paulo C PC

Scientific data 20170711

Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotr ...[more]

PMID: 28696408

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