Recombinant ACHT1 from Arabidopsis thaliana: crystallization and X-ray crystallographic analysis.

ABSTRACT: Thioredoxins (Trxs) play important roles in chloroplasts by linking photosynthetic light reactions to a series of plastid functions. They execute their function by regulating the oxidation and reduction of disulfide bonds. ACHT1 (atypical cysteine/histidine-rich Trx1) is a thylakoid-associated thioredoxin-type protein found in the Arabidopsis thaliana chloroplast. Recombinant ACHT1 protein was overexpressed in Escherichia coli, purified and crystallized by the vapour-diffusion method. The crystal diffracted to 1.7?Å resolution and a complete X-ray data set was collected. Preliminary crystallographic analysis suggested that the crystals belonged to space group C2221, with unit-cell parameters a = 102.7, b = 100.6, c = 92.8?Å.

SUBMITTER: Pan W 

PROVIDER: S-EPMC5505241 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature