Crystallization and preliminary X-ray crystallographic analysis of recombinant ?-mannosidase from Aspergillus niger.

ABSTRACT: ?-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal ?-linked mannoside in various oligomeric saccharide structures. ?-Mannosidase from Aspergillus niger was expressed in Pichia pastoris and purified to clear homogeneity. ?-Mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41?Å resolution. The crystal belonged to space group P1, with unit-cell parameters a=62.37, b=69.73, c=69.90?Å, ?=108.20, ?=101.51, ?=103.20°. The parameters derived from the data collection indicate the presence of one molecule in the asymmetric unit.

SUBMITTER: Demo G 

PROVIDER: S-EPMC3606576 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature