Ontology highlight
ABSTRACT:
SUBMITTER: Chen X
PROVIDER: S-EPMC5509561 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Chen Xingqi X Shen Ying Y Draper Will W Buenrostro Jason D JD Litzenburger Ulrike U Cho Seung Woo SW Satpathy Ansuman T AT Carter Ava C AC Ghosh Rajarshi P RP Ghosh Rajarshi P RP East-Seletsky Alexandra A Doudna Jennifer A JA Greenleaf William J WJ Liphardt Jan T JT Chang Howard Y HY
Nature methods 20161017 12
Spatial organization of the genome plays a central role in gene expression, DNA replication, and repair. But current epigenomic approaches largely map DNA regulatory elements outside of the native context of the nucleus. Here we report assay of transposase-accessible chromatin with visualization (ATAC-see), a transposase-mediated imaging technology that employs direct imaging of the accessible genome in situ, cell sorting, and deep sequencing to reveal the identity of the imaged elements. ATAC-s ...[more]