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First report of lily mottle virus on lily (Lilium sp.) in southern India.

ABSTRACT: Lily samples collected from commercial cut flower growing field at Nilgiris, Tamil Nadu, India were serologically tested through direct antigen coating-ELISA and dot immuno binding assay for the infection of potyvirus through potyvirus group specific polyclonal antibody. The RT-PCR using potyvirus universal degenerate primers (PNIbF1/PCPR1) showed the presence of a potyvirus and the sequencing results showed the association of lily mottle virus (LMoV). Further LMoV was confirmed by RT-PCR amplification using newly designed primer pair covering entire coat protein gene and sequence results showed 100% nucleotide identity with the LMoV reported from South Korean. To our knowledge, this is the first confirmed evidence for the occurrence of LMoV on lily in southern India.

SUBMITTER: Aravintharaj R

PROVIDER: S-EPMC5510624 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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First report of lily mottle virus on lily (Lilium sp.) in southern India.

Aravintharaj R R Balaji C G CG Nagendran K K Priyanka R R Karthikeyan G G

Virusdisease 20170602 2

Lily samples collected from commercial cut flower growing field at Nilgiris, Tamil Nadu, India were serologically tested through direct antigen coating-ELISA and dot immuno binding assay for the infection of potyvirus through potyvirus group specific polyclonal antibody. The RT-PCR using potyvirus universal degenerate primers (PNIbF1/PCPR1) showed the presence of a potyvirus and the sequencing results showed the association of lily mottle virus (LMoV). Further LMoV was confirmed by RT-PCR amplif ...[more]

PMID: 28770252

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