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Sustained Release of Hydrophilic l-ascorbic acid 2-phosphate Magnesium from Electrospun Polycaprolactone Scaffold-A Study across Blend, Coaxial, and Emulsion Electrospinning Techniques.

ABSTRACT: The purpose of this study was to achieve a sustained release of hydrophilic l-ascorbic acid 2-phosphate magnesium (ASP) from electrospun polycaprolactone (PCL) scaffolds, so as to promote the osteogenic differentiation of stem cells for bone tissue engineering (TE). ASP was loaded and electrospun together with PCL via three electrospinning techniques, i.e., coaxial, emulsion, and blend electrospinning. For blend electrospinning, binary solvent systems of dichloromethane-methanol (DCM-MeOH) and dichloromethane-dimethylformamide (DCM-DMF) were used to achieve the desired ASP release through the effect of solvent polarity and volatility. The scaffold prepared via a blend electrospinning technique with a binary solvent system of DCM-MeOH at a 7:3 ratio demonstrated a desirable, sustained ASP release profile for as long as two weeks, with minimal burst release. However, an undesirable burst release (~100%) was observed within the first 24 h for scaffolds prepared by coaxial electrospinning. Scaffolds prepared by emulsion electrospinning displayed poorer mechanical properties. Sustained releasing blend electrospun scaffold could be a good potential candidate as an ASP-eluting scaffold for bone tissue engineering.

SUBMITTER: Zhao X

PROVIDER: S-EPMC5512642 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature

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Sustained Release of Hydrophilic l-ascorbic acid 2-phosphate Magnesium from Electrospun Polycaprolactone Scaffold-A Study across Blend, Coaxial, and Emulsion Electrospinning Techniques.

Zhao Xinxin X Lui Yuan Siang YS Toh Pei Wen Jessica PWJ Loo Say Chye Joachim SCJ

Materials (Basel, Switzerland) 20141117 11

The purpose of this study was to achieve a sustained release of hydrophilic l-ascorbic acid 2-phosphate magnesium (ASP) from electrospun polycaprolactone (PCL) scaffolds, so as to promote the osteogenic differentiation of stem cells for bone tissue engineering (TE). ASP was loaded and electrospun together with PCL via three electrospinning techniques, <i>i.e.</i>, coaxial, emulsion, and blend electrospinning. For blend electrospinning, binary solvent systems of dichloromethane-methanol (DCM-MeOH ...[more]

PMID: 28788254

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Project description:BackgroundHere, electrospun fibers based on a blend of polycaprolactone (PCL), poly(ethylene glycol) (PEG), and gelatin methacryloyl (GelMA) were developed. The careful choice of this polymer combination allowed for the preparation of a biomaterial that preserved the mechanical strength of PCL, while at the same time improving the hydrophilicity of the blended material and human osteoblast maturation.MethodsThe morphology, chemical structure, wettability, and mechanical properties before and after UV photocrosslinking were evaluated. Furthermore, human osteoblasts (hFOB) were cultivated for up to 21 days on the scaffolds, and their potential to upregulate cell proliferation, alkaline phosphatase (ALP) activity, and calcium deposition were investigated.ResultsContact angle measurement results showed that the developed scaffolds presented hydrophilic properties after PEG and GelMA incorporation before (25°) and after UV photocross-linking (69°) compared to pure PCL (149°). PCL:PEG:GelMA-UV displayed a slight increase in mechanical strength (elastic modulus ~37 MPa) over PCL alone (~33 MPa). Normally, an increase in strength of fibers leads to a decrease in elongation at break, due to the material becoming less deformable and stiffer, thus leading to breaks at low strain. This behavior was observed by comparing PCL (elongation at break ~106%) and PCL:PEG:GelMA-UV (~50%). Moreover, increases in ALP activity (10-fold at day 14) and calcium deposition (1.3-fold at day 21) by hFOBs were detected after PEG and GelMA incorporation after UV photocross-linking compared to pure PCL. Ultrathin and hydrophilic fibers were obtained after PEG and GelMA incorporation after UV photocrosslinking, but the strength of PCL was maintained. Interestingly, those ultrathin fiber characteristics improved hFOB functions.ConclusionThese findings appear promising for the use of these electrospun scaffolds, based on the combination of polymers used here for numerous orthopedic applications.

Dataset Information

Sustained Release of Hydrophilic l-ascorbic acid 2-phosphate Magnesium from Electrospun Polycaprolactone Scaffold-A Study across Blend, Coaxial, and Emulsion Electrospinning Techniques.

Publications

Sustained Release of Hydrophilic l-ascorbic acid 2-phosphate Magnesium from Electrospun Polycaprolactone Scaffold-A Study across Blend, Coaxial, and Emulsion Electrospinning Techniques.

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