A sequence level model of an intact locus predicts the location and function of nonadditive enhancers.

ABSTRACT: Metazoan gene expression is controlled through the action of long stretches of noncoding DNA that contain enhancers-shorter sequences responsible for controlling a single aspect of a gene's expression pattern. Models built on thermodynamics have shown how enhancers interpret protein concentration in order to determine specific levels of gene expression, but the emergent regulatory logic of a complete regulatory locus shows qualitative and quantitative differences from isolated enhancers. Such differences may arise from steric competition limiting the quantity of DNA that can simultaneously influence the transcription machinery. We incorporated this competition into a mechanistic model of gene regulation, generated efficient algorithms for this computation, and applied it to the regulation

SUBMITTER: Barr KA 

PROVIDER: S-EPMC5513433 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature