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Evidence of Multi-Domain Morphological Structures in Living Escherichia coli.

ABSTRACT: A combination of light-microscopy and image processing was used to elaborate on the fluctuation in the width of the cylindrical part of Escherichia coli at sub-pixel-resolution, and under in vivo conditions. The mean-squared-width-difference along the axial direction of the cylindrical part of a number of bacteria was measured. The results reveal that the cylindrical part of Escherichia coli is composed of multi-domain morphological structures. The length of the domains starts at 150?nm in newborn cells, and linearly increases in length up to 300?nm in aged cells. The fluctuation in the local-cell-widths in each domain is less than the fluctuation of local-cell-widths between different domains. Local cell width correlations along the cell body occur on a length scale of less than 50?nm. This finding could be associated with the flexibility of the cell envelope in the radial versus longitudinal directions.

SUBMITTER: Tavaddod S

PROVIDER: S-EPMC5516040 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Evidence of Multi-Domain Morphological Structures in Living Escherichia coli.

Tavaddod Sharareh S Naderi-Manesh Hossein H

Scientific reports 20170718 1

A combination of light-microscopy and image processing was used to elaborate on the fluctuation in the width of the cylindrical part of Escherichia coli at sub-pixel-resolution, and under in vivo conditions. The mean-squared-width-difference along the axial direction of the cylindrical part of a number of bacteria was measured. The results reveal that the cylindrical part of Escherichia coli is composed of multi-domain morphological structures. The length of the domains starts at 150 nm in newbo ...[more]

PMID: 28720785

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