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Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.

ABSTRACT: G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. It has been shown that the 3' G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures, and the human telomeric DNA G-quadruplexes are considered attractive targets for anticancer drugs. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR and biophysical methodology used in our research group for the study of G-quadruplex structures in physiologically relevant solution and their interactions with small-molecule compounds.

SUBMITTER: Lin C

PROVIDER: S-EPMC5516791 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds.

Lin Clement C Yang Danzhou D

Methods in molecular biology (Clifton, N.J.) 20170101

G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. It has been shown that the 3' G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures, and the human telomeric DNA G-quadruplexes are considered attractive targets for anticancer drugs. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy is a powerfu ...[more]

PMID: 28324509

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G-quadruplex structures and G-quadruplex-interactive compounds.

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