Unknown

Dataset Information

0

Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli.


ABSTRACT: The dissemination of carbapenem resistance in Escherichia coli has major implications for the management of common infections. bla KPC, encoding a transmissible carbapenemase (KPC), has historically largely been associated with Klebsiella pneumoniae, a predominant plasmid (pKpQIL), and a specific transposable element (Tn4401, ~10?kb). Here we characterize the genetic features of bla KPC emergence in global E. coli, 2008-2013, using both long- and short-read whole-genome sequencing. Amongst 43/45 successfully sequenced bla KPC-E. coli strains, we identified substantial strain diversity (n?=?21 sequence types, 18% of annotated genes in the core genome); substantial plasmid diversity (?9 replicon types); and substantial bla KPC-associated, mobile genetic element (MGE) diversity (50% not within complete Tn4401 elements). We also found evidence of inter-species, regional and international plasmid spread. In several cases bla KPC was found on high copy number, small Col-like plasmids, previously associated with horizontal transmission of resistance genes in the absence of antimicrobial selection pressures. E. coli is a common human pathogen, but also a commensal in multiple environmental and animal reservoirs, and easily transmissible. The association of bla KPC with a range of MGEs previously linked to the successful spread of widely endemic resistance mechanisms (e.g. bla TEM, bla CTX-M) suggests that it may become similarly prevalent.

SUBMITTER: Stoesser N 

PROVIDER: S-EPMC5517641 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genomic epidemiology of global Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli.

Stoesser N N   Sheppard A E AE   Peirano G G   Anson L W LW   Pankhurst L L   Sebra R R   Phan H T T HTT   Kasarskis A A   Mathers A J AJ   Peto T E A TEA   Bradford P P   Motyl M R MR   Walker A S AS   Crook D W DW   Pitout J D JD  

Scientific reports 20170719 1


The dissemination of carbapenem resistance in Escherichia coli has major implications for the management of common infections. bla <sub>KPC</sub>, encoding a transmissible carbapenemase (KPC), has historically largely been associated with Klebsiella pneumoniae, a predominant plasmid (pKpQIL), and a specific transposable element (Tn4401, ~10 kb). Here we characterize the genetic features of bla <sub>KPC</sub> emergence in global E. coli, 2008-2013, using both long- and short-read whole-genome seq  ...[more]

Similar Datasets

| S-EPMC4670079 | biostudies-literature
| S-EPMC7763156 | biostudies-literature
| S-EPMC3993235 | biostudies-literature
| S-EPMC4538415 | biostudies-literature
| S-EPMC4184581 | biostudies-literature
| S-EPMC8058458 | biostudies-literature
| S-EPMC4904035 | biostudies-literature
| S-EPMC3491773 | biostudies-literature
| S-EPMC8209719 | biostudies-literature
| S-EPMC4214325 | biostudies-literature