Ontology highlight
ABSTRACT:
SUBMITTER: Miller JR
PROVIDER: S-EPMC5518131 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
BMC genomics 20170719 1
<h4>Background</h4>Long-read and short-read sequencing technologies offer competing advantages for eukaryotic genome sequencing projects. Combinations of both may be appropriate for surveys of within-species genomic variation.<h4>Methods</h4>We developed a hybrid assembly pipeline called "Alpaca" that can operate on 20X long-read coverage plus about 50X short-insert and 50X long-insert short-read coverage. To preclude collapse of tandem repeats, Alpaca relies on base-call-corrected long reads fo ...[more]