Ontology highlight
ABSTRACT:
SUBMITTER: Nevi L
PROVIDER: S-EPMC5519713 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Nevi Lorenzo L Cardinale Vincenzo V Carpino Guido G Costantini Daniele D Di Matteo Sabina S Cantafora Alfredo A Melandro Fabio F Brunelli Roberto R Bastianelli Carlo C Aliberti Camilla C Monti Marco M Bosco Daniela D Berloco Pasquale Bartolomeo PB Panici Pierluigi Benedetti PB Reid Lola L Gaudio Eugenio E Alvaro Domenico D
Scientific reports 20170720 1
Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota's Medium (KM) supplemented with 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) and 0.1% hyaluronans. Cryopreserved versus freshly isolated hBTSCs were similar in vitro with respect to self-replication, stemness traits, and multipo ...[more]