Ontology highlight
ABSTRACT:
SUBMITTER: Risso VA
PROVIDER: S-EPMC5520109 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Risso Valeria A VA Martinez-Rodriguez Sergio S Candel Adela M AM Krüger Dennis M DM Pantoja-Uceda David D Ortega-Muñoz Mariano M Santoyo-Gonzalez Francisco F Gaucher Eric A EA Kamerlin Shina C L SCL Bruix Marta M Gavira Jose A JA Sanchez-Ruiz Jose M JM
Nature communications 20170718
Protein engineering studies often suggest the emergence of completely new enzyme functionalities to be highly improbable. However, enzymes likely catalysed many different reactions already in the last universal common ancestor. Mechanisms for the emergence of completely new active sites must therefore either plausibly exist or at least have existed at the primordial protein stage. Here, we use resurrected Precambrian proteins as scaffolds for protein engineering and demonstrate that a new active ...[more]