Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin.
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ABSTRACT:
SUBMITTER: Chan KT
PROVIDER: S-EPMC5523129 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin.
Chan Keefe T KT Jones Stephen W SW Brighton Hailey E HE Bo Tao T Cochran Shelly D SD Sharpless Norman E NE Bear James E JE
Intravital 20130401 2
Multiphoton microscopy is a powerful tool that enables the visualization of fluorescently tagged tumor cells and their stromal interactions within tissues in vivo. We have developed an orthotopic model of implanting multicellular melanoma tumor spheroids into the dermis of the mouse ear skin without the requirement for invasive surgery. Here, we demonstrate the utility of this approach to observe the primary tumor, single cell actin dynamics, and tumor-associated vasculature. These methods can b ...[more]
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