Association of PPAR Alpha Intron 7 G/C, PPAR Gamma 2 Pro12Ala, and C161T Polymorphisms with Serum Fetuin-A Concentrations.

ABSTRACT: BACKGROUND:Both peroxisome activator proteins (PPARs) and fetuin-A play a role in lipid and glucose metabolism. AIMS:We investigated whether PPAR? intron 7 G2468/C and PPAR?2 Pro12Ala and PPAR? exon 6 C161T polymorphisms are associated with serum fetuin-A concentrations. PATIENTS AND METHODS:The PPAR? intron 7 G/C polymorphism was studied in cohort 1 (79 reference individuals, 165 postinfarction patients). The two PPAR? polymorphisms were investigated in cohort 2 (162 reference individuals, 165 postinfarction patients). Fetuin-A levels and PPAR polymorphisms were determined by radial immunodiffusion and polymerase chain reaction-restriction fragment length polymorphism techniques. RESULTS:The C allele variant of PPAR? intron 7 G2467C was associated with higher fetuin-A levels (p = 0.018). Postinfarction status (p = 0.001), PPAR? intron 7 GG/GC/CC genotypes (p = 0.032), and the C allele (p = 0.021) were the strongest determinants of fetuin-A concentration in a multiple regression model. Higher fetuin-A levels were associated with the Pro variant of PPAR?2 (p = 0.047). Postinfarction status (p = 0.041) and BMI (p < 0.001) but not PPAR?2 Pro were the strongest determinants of fetuin-A concentrations. PPAR? exon 6 C161T genotypes were not associated with fetuin-A levels. CONCLUSIONS:Fetuin-A was determined mainly by the PPAR? intron 7C allele and postinfarction status in cohort 1 and the BMI and postinfarction in cohort 2. The PPAR? intron 7C and PPAR?2 Pro variants are associated with fetuin-A levels.

SUBMITTER: Markus B 

PROVIDER: S-EPMC5525087 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature