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A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout.

ABSTRACT: We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.

SUBMITTER: Erard N

PROVIDER: S-EPMC5526787 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout.

Erard Nicolas N Knott Simon R V SRV Hannon Gregory J GJ

Molecular cell 20170701 2

We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at ...[more]

PMID: 28732207

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